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Alejandro Cohen

PhD (Memorial University)


Adjunct Professor

Scientific Director, Proteomics and Metabolomics Core Facility
Department member since 2016

LSRI N105
902-494-8359
alejandro.cohen@dal.ca
Lab Homepage

Research:

Development and application of mass spectrometry techniques in proteomics and metabolomics


Research Areas

Mass spectrometry

- Electrospray ionization (ESI)
- Atmospheric pressure chemical ionization (APCI)
- High resolution analysis (Orbitrap analyzer)
- Differential ion mobility spectrometry (DMS)
- Tandem mass spectrometry (MS/MS-MSn)
- Selected reaction monitoring (SRM/MRM)
- Collision induced dissociation (CID/CAD/HCD)
- Electron transfer dissociation (ETD)
- Data acquisition and analysis (Analyst, XCalibur)

Proteomics

- Intact protein analysis
- 1D & 2D LC-peptide fractionation
- Shot-gun proteomics
- Gel-based proteomics
- Targeted (SRM/MRM) & untargeted proteomics
- Labeling chemistry (TMT, iTRAQ, dimethylation, SILAC)
- Label free proteomics
- Database searching
- Bioinformatics (Proteome Discoverer, MaxQuant, Mascot, TransProteomicPipeline, XTandem)
- SRM Data analysis (Multiquant, Skyline)
- De-novo sequencing (Pepnovo, RapidNovor)
- Post translational modifications (Ubiquitination, Phosphorylation etc)

Metabolomics

- Targeted and untargeted metabolomics
- Lipid analysis
- XCMS data analysis

Method Development

- Customized analysis of known/unknown targets by LC-MS/MS

Keywords:
Mass spectrometry, Proteomics, Metabolomics, Bioinformatics

Publications

  1. Alejandro Martin Cohen, Axel Juan Soto and James P Fawcett, (2016) Determination of Flow Rates in Capillary Liquid Chromatography Coupled to a Nano Electrospray Source using Droplet Image Analysis Software Analytical Chemistry 88 (15):7476-7480
  2. Kunitoshi Uchida, Lusine Demirkhanyan, Swapna Asuthkar, Alejandro Martin Cohen, Makoto Tominaga, Eleonora Zakharian, (2016) Stimulation-dependent gating of TRPM3 channel in planar lipid bilayers The FASEB Journal 30(3):1306-1316
  3. Swapna Asuthkar, Alejandro Martin Cohen Lusine Demirkhanyan, Eleonora Zakharian, (2016) Ubiquitin-Mediated TRPM8 Protein Degradation in the Pathogenesis of Prostate Cancer Biophysical Journal 3(110):613a
  4. Lusine Demirkhanyan, Kunitoshi Uchida, Swapna Asuthkar, Alejandro Martin Cohen, Makoto Tominaga, Eleonora Zakharian, (2016) TRPM3 Exhibits Slight Temperature Sensitivity in the Planar Lipid Bilayer System and Requires the Presence of PIP2 Biophysical Journal 3(110):612a
  5. Lauren Davey,Alejandro Martin Cohen, Jason LeBlanc, Scott A Halperin, Song F Lee, (2016) The disulfide oxidoreductase SdbA is active in Streptococcus gordonii using a single C‐terminal cysteine of the CXXC motif Molecular microbiology 99(2):236-253
  6. Catherine Béliveau, Alejandro Martin Cohen, Don Stewart, Georges Periquet, Abdelmadjid Djoumad, Lisa Kuhn, Don Stoltz, Brian Boyle, Anne-Nathalie Volkoff, Elisabeth A Herniou, Jean-Michel Drezen, Michel Cusson, (2015) Genomic and Proteomic Analyses Indicate that Banchine and Campoplegine Polydnaviruses Have Similar, if Not Identical, Viral Ancestors American Society for Microbiology 89(17):8909-8921
  7. Pia A Elustondo, Alexander Negoda, Alejandro Martin Cohen, Evgeny Pavlov, (2015) Activation of the Mitochondrial Permeability Transition Pore Leads to the Increase in Amount of C-Subunit of ATP Synthase Associated with Channel-Forming Complex of Polyhydroxybutyrate and Inorganic Polyphosphate Biophysical Journal 108(2):608a
  8. H Awad, MJ Stoudemayer, L Usher, IJ Amster, Alejandro Martin Cohen, U Das, RM Whittal, J Dimmock, A El‐Aneed, (2014) The unexpected formation of [M− H]+ species during MALDI and dopant‐free APPI MS analysis of novel antineoplastic curcumin analogues Journal of Mass Spectrometry 49(11):1139-1147
  9. Chike Cao, Yevgen Yudin, Yann Bikard, Wei Chen, Tong Liu, Hong Li, Dieter Jendrossek, Alejandro Martin Cohen, Evgeny Pavlov, Tibor Rohacs, Eleonora Zakharian, (2013) Polyester modification of the mammalian TRPM8 channel protein: implications for structure and function Cell reports 4(2):302-315
  10. Alejandro Martin Cohen, Edwin Wang, Kenneth A Chisholm, Ripsik Kostyleva, Maureen O'Connor-McCourt, Devanand M Pinto, (2013) A mass spectrometry-based plasma protein panel targeting the tumor microenvironment in patients with breast cancer Journal of proteomics 81:135-147
  11. Pia A Elustondo, Alejandro Martin Cohen, Michał Kawalec, Michał Michalak, Elena Dedkova, Piotr Kurcok, Evgeny Pavlov, (2013) Polyhydroxybutyrate derivative induces cyclosporin a sensitive mitochondrial depolarization in mammalian cultured cells Biophysical Journal 104(2):660a
  12. RM Rideout, MJ Morgan, Y Lambert, Alejandro Martin Cohen, JH Banoub, M Treble, (2012) Oocyte development and vitellogenin production in Northwest Atlantic Greenland halibut Reinhardtius hippoglossoides J. Northw. Atl. Fish. Sci 44:15-29
  13. Alejandro Martin Cohen, Ripsik Kostyleva, Kenneth A Chisholm, Devanand M Pinto, (2012) Iodination on tyrosine residues during oxidation with sodium periodate in solid phase extraction of N-linked glycopeptides Journal of The American Society for Mass Spectrometry 23(1):68-75
  14. Alejandro Martin Cohen, Joseph H Banoub, (2011) Application of Mass Spectrometry for the Analysis of Vitellogenin, a Unique Biomarker for Xenobiotic Compounds Detection of Biological Agents for the Prevention of Bioterrorism, Springer Netherlands :301-318
  15. Joseph H Banoub, Anas El Aneed, Alejandro Martin Cohen, Nicolas Joly, (2010) Structural investigation of bacterial lipopolysaccharides by mass spectrometry and tandem mass spectrometry Mass spectrometry reviews 29(4):606-650
  16. Alejandro Martin Cohen, Farid Jahouh, Salim Sioud, Rick M Rideout, M Joanne Morgan, Joseph H Banoub, (2009) Quantification of Greenland halibut serum vitellogenin: a trip from the deep sea to the mass spectrometer Rapid Communications in Mass Spectrometry 23(7):1049-1060
  17. Anas El-Aneed, Alejandro Martin Cohen, Joseph Banoub, (2009) Mass spectrometry, review of the basics: electrospray, MALDI, and commonly used mass analyzers Applied Spectroscopy Reviews 44(3):210-230
  18. Nicolas Joly, Celine Vaillant, Alejandro Martin Cohen, Patrick Martin, Mokhtar El Essassi, Mohamed Massoui, Joseph Banoub, (2007) Structural determination of the novel fragmentation routes of zwitteronic morphine opiate antagonists naloxonazine and naloxone hydrochlorides using electrospray ionization tandem mass spectrometry Rapid communications in mass spectrometry 21(6):1062-1074
  19. Alejandro Martin Cohen, Atef AH Mansour, Joseph H Banoub, (2006) Absolute quantification of Atlantic salmon and rainbow trout vitellogenin by the ‘signature peptide’approach using electrospray ionization QqToF tandem mass spectrometry Journal of mass spectrometry 41(5):646-658
  20. Alejandro Martin Cohen, Atef AH Mansour, Joseph H Banoub, (2005) ‘De novo’sequencing of Atlantic cod vitellogenin tryptic peptides by matrix‐assisted laser desorption/ionization quadrupole time‐of‐flight tandem mass spectrometry: similarities with haddock vitellogenin Rapid communications in mass spectrometry 19(17):2454-2460
  21. Joseph Banoub, Paul Boullanger, Dominique Lafont, Alejandro Martin Cohen, Anas El Aneed, Elizabeth Rowlands, (2005) In situ formation of C-glycosides during electrospray ionization tandem mass spectrometry of a series of synthetic amphiphilic cholesteryl polyethoxy neoglycolipids containing N-acetyl-D-glucosamine Journal of the American Society for Mass Spectrometry 16(4):565-570
  22. Joseph Banoub, Anas El Aneed, Alejandro Martin Cohen, Patrick Martin, (2004) Characterization of the O-4 phosphorylated and O-5 substituted Kdo reducing end group and sequencing of the core oligosaccharide of Aeromonas salmonicida ssp salmonicida lipopolysaccharide using tandem mass spectrometry European Journal of Mass Spectrometry 10(5):715-730
  23. Joseph Banoub, Alejandro Martin Cohen, Anas El Aneed, Vincent LeQuart, Patrick Martin, (2004) Structural reinvestigation of the coreoligosaccharide of a mutant form of Aeromonas salmonicida lipopolysaccharide containing an O-4 phosphorylated and O-5 substituted Kdoreducing end group using European Journal of Mass Spectrometry 10(4):541-554
  24. Joseph Banoub, Alejandro Martin Cohen, Atef Mansour, Pierre Thibault, (2004) Characterization and de novo sequencing of Atlantic salmon vitellogenin protein by electrospray tandem and matrix-assisted laser desorption/ionization mass spectrometry European Journal of Mass Spectrometry 10(1):121
  25. Leandro CA Cerchietti, Alfredo H Navigante, Miguel W Körte, Alejandro Martin Cohen, Patricia N Quiroga, Edda C Villaamil, Marcelo R Bonomi, Berta M Roth, (2003) Potential utility of the peripheral analgesic properties of morphine in stomatitis-related pain: a pilot study Pain 105(1):265-273