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Roberto de Antueno


Senior Research Associate
Supervisor: Roy Duncan
Tupper 7K
ARJDE@dal.ca


Keywords

I have extensive experience in lipid chemistry, animal tumour models, liposomes, peptide and protein purification, and research

Education

Chemist (BSc)
La Plata National University, Argentina

Licenciate In Biochemistry (MSc) (1976)
La Plata National University, Argentina

Dr. in Biochemistry (PhD) (1980)
La Plata National University, Argentina
Study of biological and biochemical parameters of ascites Sarcoma 180 grown in essential fatty acid deficient mice

Transferable Skills & Experience

Related Experience


Adjunct Professor (2000-2003)
St. Mary's University. Halifax. Canada

Assistant Professor (1986-1991)
School of Veterinary Medicine. National University of La Plata . Argentina.

Research Associate (2001 cont)
Fusogenix Inc. & Dept. of Microbiology and Immunology. Dalhousie University. Halifax, Canada

Research Scientist (1989 -2001 )
Scotia Research Institute & QuantaNova Canada . Nova Scotia, Canada

Research Associate (1981-1984)
Lipid Research Lab. Dept. Biochem. & Biophys. Texas A&M University, USA.

Instructor (Seminar and Lab. Biochemistry Course & Post-Grad. Training in the Biophys. and Chem. of Membranes for Latin American Fellows). (1984-1986 )
School of Medicine & PNUD/UNESCO. School of Medicine National University of La Plata, Argentina.

Awards & Professional Activities

Professional Activities


A fusogenic intracellular liposome delivery system based on a fusion-associated small transmembrane (FAST) protein. R. de Antueno, D.Top, J. Corcoran,S. Salsman, J. Mader, D. Hoskin and R. Duncan. Conference on Liposomes: Drug delivery vehicles and models of biological membranes. Mc Master University, Hamilton. Ontario, Canada. (2003),

Reptilian reovirus fusion-associated small transmembrane (FAST) protein p14 facilitates liposome-mediated cargo delivery inside cells. D.Top, R. de Antueno, J. Mader, D. Hoskin and R. Duncan. 22nd Annual Meeting of the American Society for Virology. Davis, CA. USA. (2003)

Targeted fusogenic liposomes for anti-vascular cancer therapy. R. de Antueno, D. Top and R. Duncan. Cancer Research Symposium. Cancer Care Nova Scotia. Halifax, Canada. (2005)

Functional characterization of a rat delta-5 fatty acid desaturase. S.J. Allen, R de Antueno, H. Smith, M. Chan and M. D. Winther. 24th World Congress and Exhibition of the International Society for Fat Research. Berlin , Germany . (2001)

Rapid HPLC and LC-MS methods for the analysis of p14 fusion-associated small transmembrane (FAST) protein. R. Nguyen, R. de Antueno and R. Duncan. 55th ASMS Conference on Mass Spectrometry, Indianapolis, Indiana. (2007)

The effect of the SMSIARL peptide tag and sucrose purification on p14-liposome binding in prostate tissue. M. Dobro, R. de Antueno and R. Duncan. 21st National Conference on Undergraduate Research, San Rafael, California. (2007)

Fatty acid desaturase activity correlates with gene expression in two mammalian cell lines. R. de Antueno, S. Allen, L. Knickle, M. Haardt and M. Winther. 91st Annual meeting of the American Oil Chemists' Society. San Diego, USA. (2000). Inform 11, S111, (2000)

Macrophage subsets: Contrasting roles in the development of myocardial fibrosis A. Falkenham, D. Betsch, N. Rosin, T. Lee, R. de Antueno, R Duncan, J-F Legare. J. Mol. Cel. Cardiology 74 (2014) 2014 ISHR-NAS (International Society for Heart Research, North American Section Annual Meeting, May 12-15, 2014, Miami, Florida, USA (2000)

Lipid genomics: Identification of drug targets involving essential fatty acid metabolism. M. Winther, S. Allen, R. de Antueno, M. Haardt, K. Jenkins, L. Knickle, S. Nwaka and A. Ponton. Human Genome Organization- HGM 2000 meeting. Vancouver. Canada. (2000). ()

Evaluation of polyunsaturated fatty acid supplemented diets in a transgenic mouse model. R. de Antueno, R. Cantrill, Y-S Huang, P. Redden, G. Ells, K. Gaul, M. Elliot and D. Horrobin. 9th Annual meting of the Canadian Section of the American Oil Chemists' Society. Saskatoon, Canada. (1993). (1993)

Publications

  1. Thomas, M.L., de Antueno, R., Coyle, K.M., Sultan, M., Cruickshank, B.M., Giacomantonio MA, Giacomantonio, C.A., Duncan, R., Marcato, P., (2016) Citral reduces breast tumor growth by inhibiting the cancer stem cell marker ALDH1A3. Mol Oncol. 10(9):1485-1496
  2. Key, T., Sarker, M., de Antueno, R., Rainey JK, Duncan R., (2015) The p10 FAST protein fusion peptide functions as a cystine noose to induce cholesterol-dependent liposome fusion without liposome tubulation. Biochim. Biophys. Acta. 1848:408-416.
  3. Falkenham, A., de Antueno, R., Rosin N, Betsch D, Lee TD, Duncan R, Légaré JF. , (2015) Nonclassical resident macrophages are important determinants in the development of myocardial fibrosis. Am. J. Pathol. 185 :927-942.
  4. Duncan, R., J Lewis, R J De Antueno, R-L Nesbitt, E. K. Clancy, (2014) Recombinant polypeptides for membrane fusion and uses thereof. PATENT, Assignee: Innovascreen Inc.:
  5. Richardson, A., de Antueno, R., Duncan, R., Hoskin, D.W., (2009) Intracellular delivery of bovine lactoferricin s antimicrobial core (RRWQWR) kills T-leukemia cells Biochem. Biophys. Res. Commun. 388:736-741
  6. Winther, M. D., L. C. Knickle, M. Haardt, S. J. Allen, A. Ponton, R. J. De Antueno, D. K. Jenkins, S. O. Nwaka, (2009) Methods for Screening Compounds That Modulate Lipid Metabolism. PATENT. Assignee: Xenon Genetics Inc.:
  7. Winther, M. D., L. C. Knickle, M. Haardt, S. J. Allen, A. Ponton, R. J. De Antueno, D. K. Jenkins, S. Nwaka, Y. P. Goldberg, (2008) Human Elongase Genes, Uses Thereof, and Compounds for Modulating Same. PATENT. :
  8. Mader, J.S., Richardson, A., Salsman, J., Top, D., de Antueno, R., Duncan, R., Hoskin, D.W., (2007) Bovine lactoferricin causes apoptosis in Jurkat T-leukemia cells by sequential permeabilization of the cell membrane and targeting of mitochondria. Exp. Cell. Res. 313:2634-2650
  9. Corcoran JA, Salsman J, de Antueno R, Touhami A, Jericho MH, Clancy EK, Duncan R., (2006) The p14 fusion-associated small transmembrane (FAST) protein effects membrane fusion from a subset of membrane microdomains. J Biol. Chem. 281:31778-31789
  10. Top D, de Antueno R, Salsman J, Corcoran J, Mader J, Hoskin D, Touhami A, Jericho MH, Duncan R., (2005) Liposome reconstitution of a minimal protein-mediated membrane fusion machine. EMBO J. 24:2980-2988
  11. Winther, M.D., , L. C. Knickle, M. H., S. J. Allen, A. Ponton, R. J. De Antueno, D. K. Jenkins, S.O. Nwaka, Y. P. Goldberg, (2004) Fat regulated genes, uses thereof and compounds for modulating same PATENT. :
  12. de Antueno, R.J., L. C. Knickle, H. Smith, M. Elliot, S. J. Allen, S. Nwaka and, M. D. Winther. , (2001) Multiple n-3 and n-6 polyunsaturated fatty acids are substrates for single human delta-5 and delta-6 desaturases. FEBS Lett. 509:77-80
  13. de Antueno, R. J., S. J. Allen, A. Ponton, M. D. Winther. , (2001) Activity and mRNA abundance of delta 5 and delta 6 fatty acid desaturases in two human cell lines. FEBS Lett. 491:247-251
  14. de Antueno, R., M. Bai and M. Elliot., (1999) Tissue distribution and metabolism of gamma-linolenoyl-3-eicosapentaenoyl propane diol enterally or intravenously administered to mice bearing human pancreatic carcinomas. Anticancer Res. 19:2041-2048
  15. de Antueno,R., M. Elliot, M. Bai, J. Morton, P. Quiroga, M. Smith,T. Dowell, P. Redden and D. Horrobin., (1998) Diol lipids as novel compounds for n-6 and n-3 fatty acid delivery to cancer cells. In Essential Fatty Acids and Eicosanoids. Invited Papers from the 4th International Congress. R. Riemersma.R. Armstrong, R. Kelly and R. Wilson eds. AOCS press.Champaign, Illinois, USA : 32-38.
  16. de Antueno, R., M Elliot, G Ells, P Quiroga, K Jenkins and D Horrobin. , (1997) In vivo and in vitro biotransformation of the lithium salt of gamma-linolenic acid (LiGLA) by three human carcinomas. Br. J. Cancer. 75: 1812-1818
  17. de Antueno, R.J., R.C. Cantrill, Y-S Huang, G.W. Ells, M. Elliot and D. Horrobin., (1994) Metabolism of n-6 fatty acids by NIH-3T3 cells transfected with the ras oncogene. Mol. Cell. Biochem. 139 :71-81
  18. de Antueno,R.J., M. Elliot and D. Horrobin. , (1994) Liver delta-5 and delta-6 desaturase activity differs among laboratory rat strains. Lipids 29:327-331
  19. Mills, M.E., , R. de Antueno and J. Scholey., (1994) Interactions of dietary fatty acids and cyclosporine A in the borderline hypertensive rat: Tissue fatty acids. Lipids 29:27-32
  20. de Antueno, R.J., R.C. Cantrill, Y-S Huang, M. Elliot and D. Horrobin. , (1993) Relationship between mouse liver delta-9 desaturase activity and plasma lipids. . Lipids 28:285-290
  21. de Antueno. R.J., R.C. Cantrill, G.W. Ells, M. Elliot, Y-S Huang and D. Horrobin. , (1993) Metabolism of radiolabelled 18:2n-6 and 18:3n-6 by NIH-3T3 cells and DT subclone. Anticancer Res. 13: 973-978
  22. Poisson, J-P., Huang, Y-S., Mills, D.E., De Antueno R. J., Redden, P.R., Lin, X., Narce, M., and Horrobin D.F., (1993) Effect of salt-loading and spontaneous hypertension on in vitro metabolism of [1-14C]linoleic and [2-C14]dihomogammalinolenic acids Biochem. Med. Met. Biol. 49:57-66
  23. de Antueno. R.J., Cantrill, R.C., Huang,Y-S, Raha, S.K., Elliot, M., and Horrobin, D. , (1992) Effect of n-3 and n-6 fatty acids on hepatic microsomal lipid metabolism: a time course study Mol. Cell. Biochem. 118:153-161
  24. Cantrill, R.C., Ells, G., de Antueno. R.J.,Elliot, M., Raha, S.K., and Horrobin, D. , (1992) The effect of n-6 fatty acids on normal and v-Ki ras transformed NIH-3T3 cells Anticancer Res. 12:2197-2202
  25. M. G. de Bravo, R. J. de Antueno, J. Toledo, M. E. De Tomás, O. F. Mercuri, and C. Quintans, (1991) Effects of an eicosapentaenoic and docosahexaenoic acid concentrate on a human lung carcinoma grown in nude mice Lipids 26:866-870
  26. M.I. Morano, R.J. de Antueno, G. Niedfeld and F.E. Estivariz. , (1989) Neuroendocrine alterations in nude mice with a human lung carcinoma producing pro-opiomelanocortin, corticotrophin-releasing hormone and arginine vasopressin. Clin. Endocrinol. 31:290-298
  27. R.J. de Antueno, M.G. de Bravo, J. Toledo, O. Mercuri, and M. E. De Tomas, (1989) In vitro effect of eicosapentaenoic and docosahexaenoic acids on prostaglandins E2 synthesis in a human lung carcinoma. Biochem. Int. 19:489-496
  28. R.J. de Antueno, G. Niedfeld, M. E. De Tomas, O. Mercuri, and L. Montoro. , (1988) Microsomal fatty acid desaturation and elongation in a human lung carcinoma grown in nude mice. Biochem. Int. 16:413-420
  29. R.J. de Antueno, G. Niedfeld, M. E. De Tomas, O. Mercuri, and C. Quintans., (1987) Tumor lipids and liver lipid metabolism in the model human lung carcinoma/nude mice. Biochem. Int. 14:1097-1103
  30. R. Wood, G.C. Upreti and R.J. de Antueno., (1986) A comparison of lipids from liver and hepatoma subcellular membranes. Lipids 21:292-300
  31. G.C. Upreti, R.J. de Antueno and R. Wood., (1983) Membrane lipids of hepatic tissue. I. Neutral Lipids from subcellular fractions of liver and hepatoma 7288CTC. J. Nat. Cancer. Inst. 70:559-566
  32. G.C. Upreti, R.J. de Antueno and R. Wood., (1983) Membrane lipids of hepatic tissue. I. Phospholipids from subcellular fractions of liver and hepatoma 7288CTC. J. Nat. Cancer. Inst. . 70:567-573
  33. O. Mercuri, M.E. De Tomas, R.J. de Antueno., (1981) In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells. Lipids 16:893-896